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KMID : 1094720100150010167
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 1 p.167 ~ p.172
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Widdrol from Juniperus chinensis Induces Apoptosis in Human Colon Adenocarcinoma HT29 Cells
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Kwon Hyun-Ju
Lee Eun-Woo
Hong Yun-Kyung
Yun Hee-Jung
Kim Byung-Woo
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Abstract
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Juniperus chinensis is a well-known folk remedy in Korea, shown to exhibit anti-tumor, anti-bacterial, anti-fungal, abortifacient, anti-platelet, vasorelaxing, and anti-viral activities. In this study, we report the cytotoxic activity as well as the apoptotic mechanism of widdrol extracted from Juniperus chinensis against the human colon adeno-carcinoma cell line HT29. Treatment of HT29 cells with various concentrations of widdrol inhibited growth and induced apoptosis in a dose-dependent manner, as determined by cell viability, chromatin condensation, and sub-G1 phase accumulation. Western blot analysis revealed that apoptosis of HT29 cells was associated with release of cytochrome C from mitochondria into the cytosol, activation of caspases-3, -8, and -9, and proteolytic cleavage of PARP. These results suggest that widdrol exhibits an anti-proliferative effect on HT29 cells via apoptosis and can be a potential candidate in the field of anti-cancer drug discovery.
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KEYWORD
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plant widdrol, Juniperus chinensis, apoptosis, HT29 cell, anti-cancer, anti-proliferative activity
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